IPSC Genome Editing

Nowadays, the application of custom-engineered sequence-specific nucleases enables genetic changes in human cells to be easily made with much greater efficiency and precision, such as CRISPR/Cas9, and TALEN. Engineered double-stranded DNA breaks are able to efficiently disrupt genes, also which can be realized by the right donor vector, engineer point mutations, as well as gene insertions. Nevertheless, in order to ensure the maximum gene targeting efficiency and specificity, a great variety of design considerations should be taken into account. It is especially important when engineering induced pluripotent stem cells (iPSCs), because they are more difficult to transfect and less resilient to DNA damage than immortalized tumor cell lines. With years of exploration in iPSC development, Creative Biolabs is dedicated to providing a full range of iPSC genome editing services for customers all over the world.